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Exhaust gas treatment laboratory

Exhaust gas treatment laboratory

  • Categories:R & D Center
  • Time of issue:2020-06-12 00:00:00
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In terms of "odor gas treatment technology", our company has signed the "Industry-University-Research Cooperation Agreement on Odor Gas Treatment Scientific and Technological Achievements" with the School of Environmental Science and Engineering of Tianjin University. With the technical support of the expert team of the School of Environmental Science and Engineering of Tianjin University, relying on its experimental center to improve the construction of its own biological laboratory.

 

1. Main laboratory instruments

 

Name Model Manufacturer
Grating spectrophotometer 722 Shanghai Photochemical Instrument Co., Ltd.
Electro-heating standing-temperature cultivator DSX-280B  Tianjin Taisite Instrument Co., Ltd
Electric thermostatic drying oven 101A1B  Tianjin Taisite Instrument Co., Ltd
Optical microscope JA2003N  Tianjin Taisite Instrument Co., Ltd
Electronic balance HLD-3003 Changzhou Guohua Electric Appliance Co., Ltd
Thermostatic oscillator THZ-82 Changzhou Guohua Electric Co., Ltd.
Centrifugal machine TGL-16G Shanghai Anting Scientific Instrument Factory

 


2. Laboratory medicines

 

Name Type Manufacturer
Beef extract Biochemical reagent Beijing Aoboxing Biotechnology Co., Ltd.
Peptone Biochemical reagent Beijing Aoboxing Biotechnology Co., Ltd.
Agar powder Biochemical reagent Beijing Aoboxing Biotechnology Co., Ltd.
NaOH Analytically pure Tianjin Komiou Chemical Reagent Factory
H2SO4 Analytically pure Tianjin Komiou Chemical Reagent Factory
K2HPO4·3H2O Analytically pure Tianjin Dongli District Tianda Chemical Reagent Factory
C6H12O6·H2O Analytically pure Tianjin Grape Sugar Factory
NH4Cl Analytically pure Beijing Aoboxing Biotechnology Co., Ltd.
Na2S2O3·5H2O Analytically pure Beijing Aoboxing Biotechnology Co., Ltd.
MgCl2·6H2O Analytically pure Beijing Aoboxing Biotechnology Co., Ltd.
NaHCO3 Analytically pure Tianjin Komiou Chemical Reagent Factory
CaCl2 Analytically pure Tianjin Komiou Chemical Reagent Factory
MgSO4 Analytically pure Tianjin Dongli District Tianda Chemical Reagent Factory
FeSO4 Analytically pure Tianjin Dongli District Tianda Chemical Reagent Factory

 

3. Experimental method

  (1) Screening culture
 
  ①Neutral medium
 
Potassium salt, ammonium salt, magnesium salt, iron salt, calcium salt, sodium salt, phosphate, etc. were sterilized to prepare neutral liquid culture medium; Adding a certain amount of beef paste, peptone and agar powder, and sterilizing to obtain neutral solid medium.
 
  ②Acid medium
 
   Use potassium salt, ammonium salt, magnesium salt, iron salt, calcium salt, sodium salt, phosphate to prepare a solution, titrate with acid and alkali, and prepare an acidic liquid culture medium after sterilization;
 
   Adding a certain amount of beef paste, peptone and agar powder, and sterilizing to obtain acidic medium.
 
  (2) Purification and segregation
 
After the strain is activated, it is dissolved in sterile water, shaken and cultured at the corresponding temperature and rotating speed, the supernatant is continuously taken for enrichment culture, and the dominant strain is distributed, then the nutrient solution culture medium is prepared, and the single colony is cultured, and the colony characteristics such as colony size, color and transparency are observed. Then, the strains were separated and purified for many times.
 
  (3) Production culture conditions
 
  ① Calculating colony density by blood cell counting plate method and drawing bacterial growth curve;
 
  ②By changing the culture temperature, count the colonies and get the best production temperature;
 
  ③By adjusting the pH value of the culture medium, count the colonies and get the best pH value.

 

4. Experimental equipment

 

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