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Exhaust gas treatment laboratory

Exhaust gas treatment laboratory

  • Categories:R & D Center
  • Time of issue:2020-06-12 00:00:00
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In terms of "Odor Gas Treatment Technology", our company signed the "Odor Gas Treatment Technology Achievements Industry-University-Research Cooperation Agreement" with the School of Environmental Science and Engineering of Tianjin University. With the technical support of the expert team of the School of Environmental Science and Engineering of Tianjin University, relying on its experimental center to improve its own biological laboratory construction.

 

1. Main laboratory instruments

 

Name Model Factory
Grating spectrophotometer 722 Shanghai Photochemical Instrument Co., Ltd.
Heated Incubators DSX-280B Tianjin Test Instrument Co., Ltd.
Electric heating constant temperature drying oven 101A1B Tianjin Test Instrument Co., Ltd.
Optical microscope JA2003N  
Electronic balance HLD-3003 Hangzhou Youheng Weighing Equipment Co., Ltd.
Thermostatic oscillator THZ-82 Changzhou Guohua Electric Co., Ltd.
Centrifuge TGL-16G Shanghai Anting Scientific Instrument Factory

 


2. Laboratory medicines

 

Name Types of Factory
Beef extract Biochemical reagent Beijing Aoboxing Biotechnology Co., Ltd.
Peptone Biochemical reagent Beijing Aoboxing Biotechnology Co., Ltd.
Agar powder Biochemical reagent Beijing Aoboxing Biotechnology Co., Ltd.
NaOH Analytically pure Tianjin Komiou Chemical Reagent Factory
H2SO4 Analytically pure Tianjin Komiou Chemical Reagent Factory
K2HPO4·3H2O Analytically pure Tianjin Dongli District Tianda Chemical Reagent Factory
C6H12O6·H2O Analytically pure Tianjin Glucose Factory
NH4Cl Analytically pure Beijing Aoboxing Biotechnology Co., Ltd.
Na2S2O3·5H2O Analytically pure Beijing Aoboxing Biotechnology Co., Ltd.
MgCl2·6H2O Analytically pure Beijing Aoboxing Biotechnology Co., Ltd.
NaHCO3 Analytically pure Tianjin Komiou Chemical Reagent Factory
CaCl2 Analytically pure Tianjin Komiou Chemical Reagent Factory
MgSO4 Analytically pure Tianjin Dongli District Tianda Chemical Reagent Factory
FeSO4 Analytically pure Tianjin Glucose Factory

 

3. Experimental method

  (1) Screening culture
 
  ①Neutral medium
 
Use potassium salt, ammonium salt, magnesium salt, iron salt, calcium salt, sodium salt, phosphate, etc., to prepare a neutral liquid medium after sterilization; add a certain amount of beef extract, peptone and agar powder to it, sterilize Then make a neutral solid medium.
 
  ②acid medium
 
   Use potassium salt, ammonium salt, magnesium salt, iron salt, calcium salt, sodium salt, phosphate to prepare a solution, titrate with acid-base, and configure it into an acidic liquid medium after sterilization;
 
  Add a certain amount of beef extract, peptone and agar powder, and make it into an acid medium after sterilization.
 
  (2) Separation and purification
 
After the bacteria are activated, they are dissolved in sterile water, shake culture at the corresponding temperature and rotation speed, and continuously take the supernatant for enrichment culture, separate the dominant bacteria, and then prepare the nutrient medium to cultivate a single colony. Observe the colony characteristics such as colony size, color and transparency. Then streak culture, separate and purify multiple times to the dominant strain.
 
  (3) Production and cultivation conditions
 
  ① Calculate the colony density by hemocytometer method and draw the bacterial growth curve;
 
  ②By changing the culture temperature, count the colonies to obtain the best production temperature;
 
  ③By adjusting the pH value of the medium, count the colonies to obtain the optimal pH value.

 

4. Experimental equipment

 

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